type iis restriction enzyme sapi (New England Biolabs)

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New England Biolabs type iis restriction enzyme sapi
Type Iis Restriction Enzyme Sapi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 933 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/type+iis+restriction+enzyme+sapi/SapI/10__3389_slash_fsybi__2025__1619871-61-21-26
Average 97 stars, based on 933 article reviews

type iis restriction enzyme sapi - by Bioz Stars, 2026-09

97/100 stars

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Sequencing:

Article Title: Reverse Genetics with a Full-Length Infectious cDNA Clone of Bovine Torovirus.
Article Snippet: .. To delete this sequence in vitro, the type IIS restriction enzyme SapI (New England Biolabs), which recognizes asymmetric DNA sequences and cleave outsides their 59-GCTCTTCN;NNN -39 recognition site, was used. ..

Article Title: Reverse Genetics with a Full-length Infectious cDNA Clone of Bovine Torovirus
Article Snippet: .. To delete this sequence in vitro, type IIS restriction enzyme SapI (New England Biolabs), which recognizes asymmetric DNA sequences and cleave outsides their 5’-GCTCTTCN↓NNN -3’ recognition site, was used. .. First, two SapI sites at nt (4,526) and (5,736) near or in the SopA gene of BAC plasmid were removed by introducing single mutations, 5’-(4,526) GCTCTTC → C CTCTTC and 5’-(5,736) GCTCTTC → G G TCTTC respectively, using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) and two NgoMVI restriction sites located outside the two SapI sites at nt (3,633) and (7,549).

In Vitro:

Gel Purification:

Article Title: Selection for constrained peptides that bind to a single target protein
Article Snippet: The pap library was amplified from pEG03_283 using degenerate oligonucleotides oAMK-915/916 (IDT). .. Gel purification was used to isolate the 124 bp amplicon, which was then cloned into pAMK-267 using the type IIS restriction enzyme SapI (NEB). .. Linear insert and plasmid were mixed at a 1:1 molar ratio (200 fmol each) along with 10 μl 10× DNA ligase buffer, 2 μl T4 DNA ligase (HC) (20 U/μl) (M1794, Promega, WI) and 4 μl SapI in 100 μl total volume.

Amplification:

Clone Assay:

Cloning:

Article Title: Insight into Lipopolysaccharide Translocation by Cryo-EM structures of a LptDE Transporter in Complex with Pro-Macrobodies
Article Snippet: .. The insert is fused during cloning through an overlapping proline-encoding CCG codon introduced by reverse and forward primers at the 3’- and 5’-end of the sybodies and MBP respectively and released by digestion with the Type IIS restriction enzyme SapI (NEB). ..

Article Title: Cryo-EM structures of a LptDE transporter in complex with Pro-macrobodies offer insight into lipopolysaccharide translocation
Article Snippet: .. The insert is fused during cloning through an overlapping proline-encoding CCG codon introduced by reverse and forward primers at the 3′- and 5′-end of the sybodies and MBP, respectively, and released by digestion with the type IIS restriction enzyme SapI (NEB). ..

Review